Workshops

Collaborate with our technical experts. Attend our workshops to learn the latest advances in automation.

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GeneArt arrayed lentiviral CRISPR library as powerful high-throughput loss-of-function screening tools

The CRISPR-Cas9 genome editing system is a powerful tool for genetic screens to identify potential genes that are involved in various biological processes in mammalian cell culture. As of today, all the screening reports using CRISPR libraries have been performed only in a pooled format. We have developed a high-throughput production process to construct arrayed lentiviral CRISPR libraries with 4 sequence-verified distinct gRNA constructs per gene per well in a 96-well format. The gRNAs are designed to target primarily 5’ coding exons using our proprietary gRNA design tool, attempting to maximize gene-knockout and minimize off-target effects. This arrayed format allows for the much more controlled delivery of gRNA per well, and eliminating the time-consuming deconvolution steps post-screening. The pre-made lentiviral library particles, when co-infected with lentiviral particles expressing Cas9, or alternatively in Cas9 stable expression cell lines, enable high-throughput loss-of-function screens using positive or negative seralection strategies in a wide range of mammalian cell types, including primary and non-dividing cells. This new reverse genetic screening tool will enhance our ability for target discovery and validation in health and disease research and provide important information between genetic architecture and phenotypes.

Speaker: Jian-Ping Yang Ph.D., Thermo Fisher Scientific
Date: Monday, January 25th, 12:30pm

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Capture Diverse Workflows in Screening, Synthetic Biology and Clinical Genomics

One software, endless applications! Learn from three Industry leading researchers, how Thermo Scientific Momentum solutions have successfully enabled a diverse range of workflows in including secondary screening, synthetic biology, and clinical genomics.

Speakers: Dr. Huimin Zhao, Ph.D, Centennial Endowed Chair Professor of Chemical and Biomolecular Engineering at the University of Illinois at Urbana-Champaign (UIUC); Dr. Alexander Wolf, Ph.D, Scientist Assay Development and Screening, Lead Discovery Center GmbH; and Robyn Loverro, M.S., Senior Automation Scientist II, Genomic Health
Date: Monday, January 25th, 12:30pm - 1:15pm

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How innovative microplate reader features can improve your luminometric assay performance

In this workshop learn about the many luminometric assays, their benefits and pitfalls, and innovative features of microplate readers to improve your results. When selecting the best assay for your research, you need to understand the application characteristics, sensitivity requirements, throughput needs and the instrumentation available, which can be a complicated task. Learn how to select the optimal luminometric assay and how to get the results you need.

Speaker: Marika Raitio, Application Scientist, M. Sci, Thermo Fisher Scientific
Date: Tuesday, January 26th, 9:30am - 10:15am